Figure 6.

Nrf2 is required for Foxp3 differentiation. (A) Naive CD4+ T cells were isolated from the spleen and lymph nodes of WT C57BL/6 mice and activated under polarizing conditions to generate the indicated T-helper subset. The RT-qPCR was performed to determine the expression of Nrf2 mRNA. (B) Naïve CD4+ T cells induced under Treg polarization conditions were processed to validate the expression of Nrf2 mRNA by utilizing RT-qPCR. (C) Hela cells were transfected with Foxp3 or NFE2L2 plasmids. Representative confocal microscopy images were taken 48 hours after transfection, visualizing for Foxp3 (red), Nrf2 (green), and DAIP (blue). Shown are representative of three different biological replicate experiments. (D) Nrf2-deficient CD4 cells were isolated from the WT or Nrf2^fl/flCD4^cre mice and induced under iTreg conditions to validate the difference in the Treg population between the two groups. Data is shown as the Mean ± SEM.