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. 2024 Apr 22;15:1370564. doi: 10.3389/fimmu.2024.1370564

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Copyright © 2024 Jangra, Lamoot, Singh, Laghlali, Chen, Ye, García-Sastre, De Geest and Schotsaert

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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S-Ac7-Dog (− or SDI) and K-ac7-Dsa (− or SDI) in combination with IMDQ-PEG-Chol define IgG subtype profile. (A) Study outline. (B) Graphs showing ELISA end point titers calculated based on OD 450 ELISA values against serum dilutions for total IgG, IgG1, and IgG2a (n = 5 per group), represented as geometric mean. The statistical analysis was performed using one-way ANOVA with a Dunnett’s multiple comparison test, and the p-values shown are calculated in reference to the unadjuvanted QIV group, which received neither adjuvant nor LNP formulations. (C) Heatmap showing the ratio of end point titers of IgG2a to IgG1 and represented as geometric mean of IgG2a/IgG1 ratios for all animals in each group.