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. 2000 Sep 1;28(17):3233–3239. doi: 10.1093/nar/28.17.3233

Figure 2.

Figure 2

Acetylation status of histones H3 and H4 in the BRCA1 and GAPDH promoters in UACC3199, MCF7 and HMEC. Chromatin immunoprecipitations using acetyl-histone H3 and H4 antibodies were performed on UACC3199, MCF7 and HMEC. Following isolation of the acetyl-histone H3 and H4 enriched fraction of genomic DNA, the BRCA1 or GAPDH promoters were PCR amplified. Presence of a PCR product indicates acetylation of the immunoprecipitated histone. Acetylation status of histone H3 (A) or histone H4 (B) for the BRCA1 (top) and GAPDH (bottom) promoters in UACC3199, MCF7 and HMEC is shown. The different cell types analyzed are shown across the top and are grouped according to their incubation with acetyl-histone H3 or H4 antibody (+H3 Ab or +H4 Ab), no acetyl-histone antibody (–H3 Ab or –H4 Ab), or control sample. These experiments were performed three times each with similar results.