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. 2024 Apr 21;84:101945. doi: 10.1016/j.molmet.2024.101945

Figure 2.

Figure 2

GIP-Cre::hM3Dq activation significantly reduces food intake. (A) Food intake of GIP-Dq mice 1hr post ipgtt (n = 15 per treatment). (B) Overnight fast (16 h)-refeeding(1hr) food intake of GIP-Dq mice treated with VEH/CNO (n = 12–15 per treatment). (C) Food intake of ad lid fed GIP-Dq mice treated with VEH/CNO (at 1 mg/kg BW ip) at the onset of the dark phase (n = 5–6 per treatment, treatment F(1,8) = 20.66, p = 0.0042, time F(1.628, 13.03) = 180.6, p < 0.0001, interaction F(2,16) = 28.31, p < 0.0001). (D) HPM intake of ad lib fed mice treated with CNO at the onset of the dark phase (n = 8 per treatment). (E) Food intake (interaction F(2,50) = 5.578, p = 0.0065. Post hoc p = 0.001), (F) Total meal duration (treatment F(1,26) = 6.007), p = 0.0213, time F(1.933,50.27) = 4.181, p = 0.022. Post hoc p = 0.0225 (G) Inter-meal interval (interaction F(2,76) = 5.269, p = 0.0072, time F(1.659,63.02) = 16.88, p < 0.0001). Post hoc p = 0.0001), (H) Cumulative food intake (treatment F(1,25)] = 4.775, p = 0.0385, time F(2.093,52.33) = 178.6, p < 0.0001, (I) RER (interaction F(24,610) = 2.033, p = 0.0027, time F(6.816,173.2) = 42.37, p < 0.0001), (J,K) Energy expenditure (time F(9.234,234.7) = 25.67, p < 0.0001), (L) Ambulatory activity (time F(7.607, 193.3) = 13.30, p < 0.0001) and (M) body weight change of ad lib fed GIP-Dq mice treated with VEH/CNO at the onset of the dark phase (n = 14 per treatment, interaction F(1,26) = 4.242, p = 0.0496. Post hoc p = 0.0189). Values are presented as group mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 by paired (A, D) and students T test (B) and two-way ANOVA (C, E-J, L,M) and ANCOVA (body weight as covariate, K).