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Nissl-stained motor neurons in brainstem nuclei in SOD mice. Representative Nissl-stained sections of brainstem nuclei from WT and SOD mice in different stages of ALS disease, showing motor neurons in oculomotor (OCM; a-f), facial (g–l) and hypoglossal (HYPO; m-r) nuclei. Note the substantial reduction in the number of motor neurons at 16 weeks in facial (l) and hypoglossal (r) nuclei of SOD mice compared to their WT littermates (i and o, respectively). Calibration bars: 100 μm.
