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. 2023 Dec 6;15(12):mjad078. doi: 10.1093/jmcb/mjad078

Figure 5.

Figure 5

Notch and JAK/STAT signaling pathways contribute to the tumorigenic growth of dNKAP-knockdown tissues. (A–D’) Upregulation of vg(BE)-lacZ (A–B’) and 10×STAT-GFP (C–D’) in dNKAP-knockdown wing discs. Shown are single confocal sections of third-instar wing imaginal discs with posterior side to the right and dorsal side up. Scale bar, 50 μm. (E) Quantification of vg(BE)-lacZ signals from A and B. n = 6. Note that three different regions with the same size were calculated per disc. (F) Quantification of 10×STAT-GFP signals from C and D. n = 9. Note that three different regions with the same size were calculated per disc. (G–N’) Reducing Notch or JAK/STAT signaling, but not JNK signaling, suppresses tumorigenic growth of dNKAP-knockdown wing discs. Shown are single confocal sections of third instar wing imaginal discs with dorsal side up. Scale bar, 100 μm. (O) Quantification of GFP-positive area in wing discs with the indicated genotypes. n = 12, 14, 14, 13, 9, 10, 8, and 15 for each genotype, respectively. The value was calculated by normalizing to the mean area of the control discs. (P) Knockdown of Notch suppresses the upregulated Socs36E level in dNKAP-depleted wing discs. Quantification of Socs36E levels in wing discs with the indicated genotypes. n = 3. GFP or lacZ was used to mark the knockdown domain. N.S. indicates no significance. *P < 0.05, **P < 0.01, ****P < 0.0001.