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Agarose gel (1%) showing the electrophoresis patterns of bacteriophage λ DNA digested by different amounts of restriction endonuclease, Hpy99II, encoded by JHP46. Lane 1, bacteriophage λ DNA + HindIII, φX174 + HaeIII, size standard; lane 2, λ DNA digested with 9 µl Hpy99I; lane 3, λ DNA digested with 3 µl Hpy99I; lane 4, λ DNA digested with 1 µl Hpy99I; lane 5, λ DNA digested with 0.3 µl Hpy99I.
