Fig. 3.

ESCC cell-derived exosomes promote macrophage M2 polarization by transferring NAT10 A. Exosomes extracted from ESCC cells were observed using TME. B. The abundant expression of exosome surface markers (CD63 and TSG101) was assessed through Western blot. C. The diameter and concentrations of exosomes were analyzed using NTA. D. Exosomes were labeled with PKH26 for tracing. E. Changes in NAT10 protein levels in ESCC cells-derived exosomes (Eca-109-Exos or TE-5-Exos) were detected through Western blot after NAT10 overexpression or interference. F. The impact of ESCC cells-derived exosomes (Eca-109-Exos or TE-5-Exos) on NAT10 expression in macrophages was measured by Western blot. G. NAT10 expression was measured in macrophages treated with exosomes derived from ESCC cells or NAT10-silenced ESCC cells (Eca-109/sh-NAT10#1-Exos or TE-5/sh-NAT10#1-Exos). H. Expression levels of all four M2 markers were assessed in macrophages treated with ESCC cells-derived exosomes or NAT10-silenced ESCC cells-derived exosomes. I. The percentage of M2 macrophages was determined by flow cytometry analysis after treatment with the four exosomes. J. The percentage of M1 macrophages was assessed by flow cytometry analysis after treatment with the four exosomes. K. The correlation between NAT10 and M1 marker CD86, as well as NAT10 and M2 marker CD163, was evaluated in tissue expression. ^⁎⁎P < 0.01.