Fig. 6.

NAT10 stabilizes FASN through ac4C modification A. NAT10 targets were screened from the GSE102113 dataset, and four of them were found to be correlated with lipid metabolism in PathCards. B. The expression of four targets in macrophages after treatment with two types of exosomes was measured by RT-qPCR. C. The expression of four targets in macrophages was measured by RT-qPCR after the overexpression of NAT10. D. Acetylation sites in FASN were predicted using PACES. E. The binding of NAT10 protein to FASN mRNA was demonstrated through RIP assay. F. The effect of NAT10 overexpression on the binding of NAT10 protein to FASN mRNA was demonstrated through RIP assay. G. The lifetime detection of FASN mRNA was performed in macrophages treated with or without NAT10 overexpression. H. The enrichment level of FASN in ac4C was determined by RIP assay in macrophages treated with ESCC cells-derived exosomes or NAT10-silenced ESCC cells-derived exosomes. I. The lifetime of FASN mRNA in macrophages treated with ESCC cells-derived exosomes or NAT10-silenced ESCC cells-derived exosomes was measured by RT-qPCR. J. The expression level of FASN in macrophages treated with ESCC cells-derived exosomes or NAT10-silenced ESCC cells-derived exosomes was measured by RT-qPCR. ^⁎⁎P < 0.01.