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. 2000 Sep 1;28(17):3370–3378. doi: 10.1093/nar/28.17.3370

Figure 1.

Figure 1

(A) Amino acid sequence of human CTCF. The first methionine is designated as M1. All other amino acids delineating selected deletions are indicated by the specific amino acid followed by its position in the sequence. The putative zinc fingers are indicated by brackets. Amino acids removed in individual zinc finger deletions are underlined. (B) Schematic representation of the CTCF molecule indicating its N-, C-terminal and zinc finger (Zn) domain. The approximate relative positions of N- and C-terminal deletions are indicated by arrows. (C) Sequence of the three oligonucleotides APBβ[WT], APBβ[–94] (derived from the APP promoter) and C-MYC used in their double-stranded form for mobility shift electrophoresis. Native APP and c-myc promoter sequences are written in capital letters and non-promoter sequences are written in lower case. The positions of nucleotides –125, –94 and –64 within the APP promoter are delineated in the APBβ oligonucleotides. In oligonucleotide APBβ[–94] the sequence upstream of position –94 is underlined, representing the exact reproduction of the sequence as it exists in expression vector APP[–94], which is derived from plasmid CAT2bGAL (26).