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. 2000 Sep 1;28(17):3370–3378. doi: 10.1093/nar/28.17.3370

Figure 7.

Figure 7

Mobility shift competition of CTCF binding to double-stranded oligonucleotide APBβ[WT]. Full-length recombinant CTCF was bound to 5′-end-labeled APBβ[WT] either without competitor (lane 1), or with a 3- (lane 2), 10- (lane 3), and 30-fold (lane 4) molar excess of unlabeled APBβ[WT], a 3- (lane 5), 10- (lane 6), 30- (lane 7), and 100-fold (lane 8) excess of unlabeled APBβ[–94], or a 10- (lane 9), 30- (lane 10) 100- (lane 11) and 400-fold (lane 12) excess of C-MYC (Fig. 4C). The amount of CTCF bound without competitor was assigned the value of 100, and all other binding activities are expressed as a fraction thereof.