Figure 2.

Single-cell transcriptional characteristic of malignant cells in IFS. (A) Violin plots depicting the expression of three selected genes (POSTN, IGFBP2, and CTHRC1) in malignant cells compared with normal cells. (B) HE and immunohistochemistry staining revealing the universal expression of selected genes in the four studied IFS tissues (20×). (C) UMAP visualizing the single-cell expression profiles of IFS malignant cells. The color indicates various cell clusters or cell subtypes. (D) Heatmap displaying the Pearson correlation coefficients calculated between the average gene expressions of malignant cell clusters. (E) Bar charts depicting the enrichment of GO terms based on the top 50 upregulated genes in various malignant cell subtypes. (F) Heatmap displaying the expression of CSC markers in malignant cell subtypes. HE, hematoxylin and eosin; UMAP, uniform manifold approximation and projection; GO, Gene Ontology; BMP, bone morphogenetic protein; CSC, cancer stem cell; IFS, infantile fibrosarcoma.