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. Author manuscript; available in PMC: 2024 May 6.
Published in final edited form as: ACS Chem Biol. 2022 Mar 21;17(4):776–784. doi: 10.1021/acschembio.2c00018

Figure 4.

Figure 4.

Effect of COPS5 inhibition using CSN5i-3 and CSN5i-3e on NSCLC and SCLC cell lines. (a) Schematic representation of regulation of cullin neddylation and corresponding substrates by COPS5. (b) Effect of COPS5/COPS6 siRNA knockdown on cell viability in H460 cells. α-tubulin (TUBA) is used as a housekeeping gene control. (c) Effect of CSN5i-3 (active enantiomer) and CSN5i-3e (inactive enantiomer) on cell viability in H460 cells. (d) Effect of CSN5i-3 and CSN5i-3e on cell viability in a panel of NSCLC and SCLC cell lines. (e) Effect on substrates of Cul1 by CSN5i-3 and CSN5i-3e. In parts c−e the cell lines showing loss of viability due to CSN5i-3 treatment are shown in red and the cell lines unaffected by CSN5i-3 treatment are shown in blue.