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. 2000 Sep 1;28(17):3206–3215. doi: 10.1093/nar/28.17.3206

Figure 1.

Figure 1

Calf mtMYH is localized in the mitochondrial matrix. Lane 2 contains the A/8-oxoG mismatch oligonucleotide only (D). One hundred micrograms of protein from the non-Proteinase K treated (lane 1, F) and Proteinase K treated Ficoll-purified mitochondria lysate (lane 3, K) were assayed for binding with A/8-oxoG-containing oligonucleotide as described in Materials and Methods. The mitochondrial lysate was fractionated using digitonin as described and each fraction was assayed for A/8-oxoG mismatch binding activity. Lanes 4–6 contain reactions which contained 100 µg of the outer membrane and inter-membrane protein fraction (lane 4, O), 100 µg of the inner membrane fraction (lane 5, I) or 100 µg of the matrix protein fraction (lane 6, M). Arrows indicate the positions of free DNA (F) and enzyme-bound DNA complex (C). Quantitation of the gel is shown in Table 1.