Table 2. Purification of mtMYH from calf liver mitochondria.

Fraction	Volume (ml)	Protein (mg)a	Specific activity		Fold purification (A/8-oxoG) binding
			Nicking (units/mg)b	Binding (units/mg)c
(I) Mitochondrial lysate	400	2700	10	200	1
(II) Ammonium sulphate	85	1040	55	900	5
(III) Phosphocellulose	20	40	1100	2800	15
(IV) ssDNA cellulose	2.5	3.1	2450	4800	25
(V) Q-Sepharose	0.5	0.4	16 250	18 400	94
(VI) Superose-12	0.3	0.1	45 000	92 700	475

^aProtein concentration was measured by Bradford assay.

^bOne unit of nicking activity is defined as that resulting in cleavage of 1% (0.018 fmol) of 3′-labeled DNA 20mer with an A/8-oxoG mismatch at 37°C for 30 min.

^cOne unit of binding activity is defined as that resulting in binding of 1% (0.018 fmol) of 3′-labeled DNA 20mer with an A/3-oxoG mismatch at 37°C for 30 min.