Fig. 5.

The proposed metric for transcription activity in TADs, $\text{RPKMT}$, is consistent with the transcription activities in different epigenetic classes of TADs identified previously by Sexton et al. [59]. Panel A: Mean (solid boxes) and median (dashed lines) transcription levels (in $\text{RPKMT}$) for Active TADs (n=494), HP1/centromeric (n=52), Null (n=492), and PcG (n=131) epigenetic TAD classes. Error bars are standard errors of the mean. Both mean and median $\text{RPKMT}$ transcription levels in Active TADs are at least 2 times greater than those in the three repressive epigenetic TAD classes (Null, PcG, and HP1/centromeric). These $\text{RPKMT}$ transcription levels demonstrate consistency with the gene transcription levels (in medians of RNA-seq averages) within each epigenetic class of TADs shown in Figure 3C of Ref. [59], reproduced in Panel B