Figure 5. Fixation of variation in sf3b4 between anadromous Dolly Varden charr and the Lake Kronotskoe species flock.

(A) Three highly differentiating synonymous variants lie within sf3b4. Fst is plotted in non-overlapping 10kb sliding windows along the length of the chromosome LG30. Sf3b4 is contained within a 200kb interval of low nucleotide diversity as illustrated by sliding window plots of Tajima’s Pi. Horizontal dotted gray line represents Fst = 0.9. The broad locus encompassing sf3b4 is shown in detail including plots of the number of variants, Fst, and Tajima’s Pi in 10kb non-overlapping sliding windows. (B) 3 synonymous variants in sf3b4 are fixed in lacustrine Dolly Varden charr; dark orange, non-reference allele fixed; light orange, non-reference allele predominant (alt. allele freq. > 50%) in sequence pool; Light blue, a lineage for which the reference allele is predominant (ref. allele freq. > 50%) at the locus.