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. 2000 Sep 1;28(17):3323–3331. doi: 10.1093/nar/28.17.3323

Figure 2.

Figure 2

Synthesis of 3′-pNP DNA. (A) The 3′-terminal phosphate of an oligonucleotide is illustrated on the left; condensation with para-nitrophenol yields 3′-pNP DNA, illustrated on the right. (B) 3′-PO4 and 3′-pNP DNA (23mer) were resolved by reverse phase chromatography (see Materials and Methods). The resulting chromatogram (A260 versus time) is shown. (C) 5′-end-labeled 23mer containing a 3′-PO42– (lane 1), 3′-pNP (lane 2), or 3′-PO42– and 3′-OH mixture (lane 3) were resolved on a 20% acrylamide, 8 M urea, 0.5× TBE (45 mM Tris–borate, 1.25 mM EDTA) 0.4 mm sequencing gel. The resulting autoradiogram is shown.