Figure 1.

snRNA-seq of the human lateral septum (LS) identifies 25 transcriptionally distinct cell populations. a, Description of workflow to dissect human LS, dissociate nuclei and perform droplet based single nucleus capture. b, t-distributed Stochastic Neighbor Embedding of 9,225 nuclei from human LS colored by cluster identity. Features plots demonstrating distribution of expression across cell types for c, SYT1, d, SNAP25, e, GAD1, f, GAD2, g, SLC17A6, h, SLC17A7, i, MOBP, and j, GFAP. k, Heatmap of expression values for general cell class marker genes used to determine cluster identity. Color of square corresponds to centered and scaled expression values (logcounts).