Figure 1.

Schematic representation of ZmMET1 gene. Organization deduced from sequences of genome (AF063403) (A) and partial cDNA (B) is illustrated. The open reading frame is represented by boxes, the positions and numbers of nucleotide introns are indicated. Several functional domains based on homology with other known sequences were identified; the I–X catalytic motifs of eukaryote METase; NLS, nuclear localization signal; acidic domain; and other basic sites are also indicated. P1 and P2 indicate the DNA fragments amplified by PCR and used as templates for the in situ hybridization and mRNA blots analysis respectively.