Figure 3.

Southern and northern blot analyses. Genomic DNA (10 µg per lane) was digested with BamHI, EcoRI, EcoRV or HindIII, separated on an agarose gel, blotted and probed with a radioactively-labeled ZmMET1 cDNA fragment. The same membrane was washed at low (A) and high stringency (B). The size of the hybridizing signals is indicated along with the positions of DNA molecular markers. Hybridizing signals observed only in blots washed under low stringency are indicated with arrowheads. (C) RNA blot analysis of transcripts of ZmMET1 and H3. RNA blot analysis was carried out with 20 µg of total RNA extracted from leaf blades, leaf sheaths, mesocotyl, mature root region and root apices of 15 day old seedlings. Hybridization was performed successively with the P2 and P3 fragments of the ZmMET1 cDNA and H3 cDNA respectively.