Figure 6.

Effects of cold and EMS on ZmMET1 and H3 transcript accumulation. Treatments were by chilling (A, B) and EMS (C, D) in mecocotyl (A, C) and root tissues (B, D). RNA analysis was carried out with 20 µg of total RNA extracted from the coleptile node and mesocotyl from 15 day old maize seedlings. After stress treatments as indicated, RNA blots were probed successively with the P2 and P3 fragments of the ZmMET1 cDNA and H3 cDNA, respectively. Results of a densitometric estimation of relative amounts of mRNA for ZmMET1 (open circles) and for H3 (closed circles) are graphically shown.