Figure 7.

Immuno-blotting assay of ZmMET1. Cold treatment was given to mesocotyl tissues for transitory period (A) and to mesocotyl and root tissues for prolonged period (B). Total protein samples prepared from maize tissues treated with cold were separated by electrophoresis (110 µg per lane) and transferred to polyvinylidene difluoride membranes. After binding of rabbit anti-ZmMET1 antibodies or pre-immune serum, locations of the antigen were visualized by using horseradish peroxydase-conjugated goat anti-rabbit antibody and the ECL reagent (Amersham Life Science), followed by exposure to X-ray film.