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PCR analysis of recombinant clones with primers LUG1 and LUG2. The parent clone (lane 1) gives a 440 bp product, while insertion of the TetR cassette into the β-globin gene yields a 1830 bp product (lane 2). Deletion of the TetR gene after a second round of recombination with a PCR fragment carrying only the IVS I-110 mutation in the β-globin gene restores the original PCR product size (lanes 3 and 4).
