Figure 3. Co-administration of ineffective doses of prazosin and propranolol reduced AOD and CLAD.

(A) Schematic experimental timeline for co-administration of ineffective doses of prazosin and propranolol. Prazosin (0.25mg/kg) or vehicle was first injected, and 10 minutes animals were injected with propranolol (2.5mg/kg) or vehicle and then 20 minutes later were exposed to AOD or CLAD drinking. (B) Systemic administration of prazosin or propranolol alone did not affect AOD or CLAD. However, co-administration of these drugs together decreased both drinking conditions. (C) Prazosin+propranolol changes in AOD and CLAD were not correlated. (D) Changes in AOD were not correlated with the basal AOD. (E) Changes on CLAD were negatively correlated with basal quinine-alcohol drinking. (F) Co-administration of prazosin and propranolol did not change the concurrent water intake. A dashed line in (C-E) indicates where drug intake was not different from vehicle, and values below the dashed line indicate that alcohol drinking under drug exposure was reduced relative to vehicle treatment. *,** p<0.05, p<0.01 for treatment or drinking condition effects, from two-way ANOVA. @ p<0.05 correlation.