Figure 5.

Plasma proteome analysis. (A) Volcano plots showing -log₁₀ P-values and log₂ fold changes from differential expression analysis between each combination. The red dots indicate the differentially expressed proteins (DEPs), and the protein names are shown. (B) Box plots showing expression levels of seven proteins that were isolated as DEPs with seronegative MGs compared to those in HC and AChR-positive MG. Numerical values show the median for each group. Red lines and asterisks show statistically significant combinations based on Tukey’s HSD test (*P < 0.05, **P < 0.005, and ***P < 0.0005). (C) Scatter plot between the expression levels of CD22 protein and the disease severity score by MGC with Pearson’s correlation coefficient. Numerical values show the correlation coefficient using 30 MG patient samples (AChR-positive MG and seronegative MG pre-/post-IVIg treated patients). (D) Scatter plot between the expression levels of CD22 protein and the frequency of B cells using Pearson’s correlation coefficient. Numerical values show the correlation coefficient using 30 MG patient samples. (E) Scatter plot between the expression levels of CD22 protein and the expression levels of CD22 genes based on the scRNA-seq data with Pearson’s correlation coefficient. Numerical values show the correlation coefficient using 30 MG patient samples. The blue line indicates the regression line, and the gray zone indicates the 95% confidence interval. MG, myasthenia gravis; HC, healthy control; IVIg, intravenous immunoglobulin; AChR, acetylcholine receptor.