Table 1. Luciferase induction measurements.
| pG-BON | pDYAL-BON | ||||
|---|---|---|---|---|---|
| Harvest | Dox (µg/ml) | ng Luc/mg protein | Fold-induction | ng Luc/mg protein | Fold-induction |
| C127 mouse cells | |||||
| 24 h | 0 | 0.15 | ∅ | 0.42 | ∅ |
| 24 h | 1 | 1.74 | 11.60 | 64.51 | 153.60 |
| 24 h | 5 | 1.93 | 12.87 | 86.87 | 206.83 |
| 48 h | 0 | 0.12 | ∅ | 0.34 | ∅ |
| 48 h | 1 | 1.38 | 11.50 | 75.97 | 223.44 |
| 48 h | 5 | 1.94 | 16.17 | 113.57 | 334.03 |
| C57 mouse cells | |||||
| 24 h | 0 | 0.03 | ∅ | 0.16 | ∅ |
| 24 h | 1 | 1.66 | 55.33 | 65.64 | 410.25 |
| 24 h | 5 | 1.52 | 50.67 | 58.31 | 364.44 |
| 48 h | 0 | 0.05 | ∅ | 0.25 | ∅ |
| 48 h | 1 | 0.58 | 11.60 | 89.40 | 357.60 |
| 48 h | 5 | 1.77 | 65.40 | 61.50 | 246.00 |
Values of luciferase were determined by using a standard curve generated with recombinant luciferase. Luciferase values were normalized to protein concentrations in the extracts. The fold-inductions in mouse cell lines C127 and C57 are also presented. The data represent the average of two independent experiments, which gave closely similar results.