Table 1.

Studies with direct manipulation of mitochondrial dynamics machinery

Model system	Mitochondrial dynamics perturbation	Major findings	References
Human mammary epithelial cells	Mdivi-1 Drp1 K38A overexpression	Drp1 inhibition decreases YAP/TAZ-dependent clonogenicity	[55]
Human primary AML and AML cell lines	Fis1 shRNA knockdown	Fis1 knockdown inhibits mitophagy, colony formation, and engraftment potential	[77]
Human glioblastoma PDX	Drp1 S616E S637A overexpression Drp1 shRNA knockdown	Drp1 S616E S637A overexpression inhibits differentiation and upregulates stemness gene expression Drp1 knockdown decreases OCR, in vitro proliferation, spherogenesis, and in vivo tumor growth	[57]
Mouse embryonic cortex development	Drp1 or Mfn1/2 knockout Opa1 shRNA knockdown	Inhibition of mitochondrial fission decreases ROS production and neural stem cell differentiation Inhibition of mitochondrial fusion promotes ROS production and differentiation	[73]
Human prostate cancer cell lines	Mff shRNA knockdown	Mff knockdown decreases OCR, cell proliferation, spherogenesis, and in vivo tumor growth	[75]
Human mammary epithelial cells Human breast cancer cell lines	Mfn1 knockdown	Mfn1 knockdown decreases stemness and tumor sphere formation	[87]
Mouse embryonic fibroblasts	Opa1 knockout	Opa1 knockout inhibits protective priming to irradiation exposure	[100]