Fig. 2.

LINC01615 preserves cell survival under starvation stress. A DLD1 cells were transfected with either a nontargeting shRNA (sh-Ctrl) or a shRNA targeting LINC01615 (sh-LINC01615), and LoVo cells were transfected with either an empty vector or a plasmid containing LINC01615. Twenty-four hours after transfection, the cells were starved for 6 h. The CCK-8 assay showed the effects of LINC01615 knockdown on proliferation in DLD1 cells without serum supplementation. B Colony formation assays were performed to examine the effects of LINC01615 knockdown on proliferation in DLD1 cells without serum supplementation. C Statistical analysis of colony formation numbers in (B). D CCK-8 assay showed the effects of LINC01615 overexpression on proliferation in LoVo cells without serum supplementation. E Colony formation assays were performed to examine the effects of LINC01615 overexpression on proliferation in LoVo cells without serum supplementation. F Statistical analysis of colony formation numbers in E. G Flow cytometry data presented as histograms showing the effects of LINC01615 knockdown on the apoptotic rate in DLD1 cells without serum supplementation. H Flow cytometry data presented as histograms showing the effects of LINC01615 overexpression on the apoptotic rate in LoVo cells without serum supplementation. I WB was used to examine the effects of LINC01615 knockdown on the expression of cleaved caspase 3, caspase 7, caspase 9 and BCL-2 in DLD1 cells without serum supplementation. J WB was used to examine the effects of LINC01615 overexpression on the expression of cleaved caspase 3, caspase 7, caspase 9 and BCL-2 in LoVo cells without serum supplementation. Data are the means ± SDs (n = 3 independent experiments), *p < 0.05, **p < 0.01