Table 2.
Average values of membrane parameters of RAW 264.7 and MEF cells measured by AFM
| Genotype | EY, Pa | FT, pN | TM, pN/μm | RT, nm | ΔP, kPa | |
|---|---|---|---|---|---|---|
|
Macrophages (RAW 264.7 cells) |
Control | 813.8 ± 26.9 | 61.7 ± 1.9 | 518.4 ± 31.1 | 11.7 ± 0.4 | 88.6 ± 5.2 |
| K3KO1 | 235.0 ± 6.1 | 39.7 ± 1.5 | 215.8 ± 18.3 | 17.0 ± 0.6 | 25.4 ± 2.1 | |
| K3KO2 | 245.2 ± 6.2 | 40.8 ± 1.4 | 220.0 ± 16.1 | 16.0 ± 0.5 | 27.5 ± 13.6 | |
| K3KO1hK3 | 615.6 ± 33.5 | 48.6 ± 1.3 | 317.1 ± 19.7 | 13.6 ± 0.3 | 46.6 ± 3.2 | |
| K3KO1hK3pxn/act | 380.8 ± 19.6 | 40.0 ± 1.2 | 209.0 ± 10.1 | 16.3 ± 0.5 | 25.6 ± 1.1 | |
| K3KO1hK3int | 504.8 ± 22.7 | 44.8 ± 1.6 | 269.1 ± 18.2 | 14.9 ± 0.5 | 36.1 ± 2.8 | |
| K3KO2hK3 | 576.9 ± 3.6 | 50.8 ± 1.3 | 334.6 ± 17.7 | 12.7 ± 0.3 | 52.7 ± 3.4 | |
| K3KO2hK3pxn/act | 402.4 ± 10.1 | 41.8 ± 1.5 | 234.8 ± 20.8 | 15.7 ± 0.4 | 29.9 ± 3.3 | |
| K3KO2hK3int | 504 ± 17.8 | 43.97 ± 1.5 | 258.5 ± 17.9 | 15.1 ± 0.5 | 34.3 ± 2.4 | |
| MEF cells | Control | 3499 ± 43 | 92.3 ± 1.22 | 1093.9 ± 28.3 | 6.9 ± 0.1 | 317 ± 8.6 |
| K2KO | 683 ± 51 | 33.9 ± 1.82 | 158.5 ± 15.9 | 20.4 ± 1.2 | 15.5 ± 1.2 | |
| K2KOhK2 | 2549 ± 41 | 62.6 ± 1.64 | 513.8 ± 30.1 | 10.3 ± 0.2 | 99.8 ± 6.7 | |
| K2KOhK2pxn/act | 1964 ± 38 | 54.8 ± 1.9 | 403.4 ± 28.6 | 12.1 ± 0.4 | 65 ± 5.5 |
Mean ± St. error of all the parameters measured by AFM for RAW and MEF cells, calculated as described in supplementary methods. The top panel shows effects of K3 deletions and re-expression of hK3 and hK3pxn/act or hK3int in RAW cells. Bottom panel shows similar analyses for K2 in MEF cells. The strong positive linear correlation between effective cell stiffness and ΔP across the membrane suggests that depletion of K2 or K3 in hematopoietic cells led to decreased internal osmotic pressure, thereby compromising the effective cell stiffness and encouraging higher deformation, with possible implications for hematopoietic cellular functions such as migration and adhesion