Fig. 6.

PGE2 activated Erk 1/2 /Beclin-1 pathway was inhibited by E7046. The expression levels of Akt, phosphorylated-Akt (p-Akt), mTOR, p-mTOR, Erk1/2, p-Erk1/2 and Beclin-1 in LX-2 cells were determined by Western blot. Cells were cultured with 100 ng/mL PGE2, in the presence or absence of 1 μM E7046 for 48 h. The intensity of blots was quantified with ImageJ software. The protein and phosphorylation levels were normalized by relative intensity. P-values were obtained by one-way ANOVA. **P < 0.01 and ***P < 0.001 compared with controls