. 2021 Apr 15;78(10):4589–4613. doi: 10.1007/s00018-021-03801-1

Table 1.

Mammalian cell lines with Ku70 or Ku80 deficiencies

Subunit	Species	Cell line/tissue	Type of cell line	Type of deficiency	Viability	Reference(s)
Ku70	Human	RERF-LC-A1	Immortal	Knockdown	Viable	[58]
		Nalm-6	Immortal	Heterozygous	Viable	[59]
		HCT116	Immortal	Heterozygous	Viable	[60]
		HCT116	Immortal	Knockout	Inviable	[60]
		HAP1	Immortal	Knockout	Inviable	[8]
		KBM7	Immortal	Knockout	Inviable	[8]
	Mouse	Mouse Embryonic Fibroblasts (MEFs)	Immortal	Knockout	Viable	[61]
		Embryonic Stem (ES) cells	Primary	Heterozygous	Viable	[16]
		Embryonic Stem (ES) cells	Primary	Knockout	Viable	[16]
		Fibroblasts	Primary	Heterozygous	Viable	[62]
		Fibroblasts	Primary	Knockout	Viable	[62]
		B and T lymphocytes, thymocytes, MEFs, spleen, bone marrow	Primary	Knockout	Viable	[63]
Ku80	Hamster	sxi-2, sxi-3	Immortal	Knockout	Viable	[64]
		CHO-xrs-6 or xrs6^a	Immortal	Knockout	Viable	[65, 66]
		CHO-xrs-4 or xrs4^a	Immortal	Knockout	Viable	[65, 66]
		CHO-xrs-5 or xrs5^a	Immortal	Knockdown	Viable	[66]
		XR-V15B	Immortal	Knockout	Viable	[67]
		XR-V9B	Immortal	Knockout	Viable	[68]
	Human	MRC5V1	Immortal	Knockdown	Viable	[69]
		HCT116	Immortal	Heterozygous	Viable	[7]
		HCT116	Immortal	Knockout	Inviable	[7]
		HeLa	Immortal	Knockdown	Reduced viability	[70]
		U2OS
		SAOS
		Nalm-6	Immortal	Heterozygous	Viable	[59]
	Mouse	MEFs	Immortal	Knockout	Viable	[6]
		Bone marrow, B and T lymphocytes, MEFs, spleen	Primary	Knockout	Viable	[6, 71]
		ES cells, fibroblasts, thymus	Primary	Knockout	Viable	[6]
		Thymocytes	Primary	Knockout	Viable	[71]

^axrs hamster cell lines can be reverted to wild-type levels of Ku80 with azacytidine treatment suggesting they may not be true knockouts, but instead contain one silenced, but functional allele [66]