Table 1. mRNA sequence flanking quadruplets.

	Plasmid name	5′-mRNA-3′	Outgoing frame
Assay constructs	pAGCC1	AGC│UUCGCCUAGAAGA.UUG│GGC	+
	pAGCC2	AGC│UUCGCCUAGAUAGAUUG│GGC	–
	pAGCC2X	AGC│UUCGCCUAGAUACAUUG│GGC	–
Protein characterization constructs	pMGCC2	G│AUCCGCGCCUAGAUAGA│UUG	–
	pMCGA2	G│AUCCAUCGAUAGAUAGA│UUG	–
	pMCGG2	G│AUCCAUCGGUAGAUAGA│UUG	–
tRNA anticodon loop		3′-AAAUCNUA-5′

The mRNA sequence of the area surrounding the tandem (or single) UAGA quadruplets is shown with the associated plasmid name. The sections of mRNA shown correspond to the DNA oligos used to generate the constructs. Italicized letters indicate bases that are part of the restriction sites used in cloning DNA oligos into the respective plasmid vectors. Vertical bars indicate incoming and outgoing reading frames relative to the 5′ and 3′ fusions, respectively. Sequences are aligned for ease of comparison within groups. The final column indicates the outgoing reading frame relative to the 5′ fusion. The bottom row shows the 3′-AUCN-5′ tRNA^Leu anticodon loop for comparison with the aligned UAGA mRNA sequences.