Fig. 2.

MDC1 regulates G2/M transition by stabilizing cyclin B1 in mouse oocytes. A, B Control (siCTL) and siMDC1-injected oocytes were treated with etoposide (ETP). The incidence of GVBD for 4 h and the developmental stage of oocytes after 24-h culture were quantified. C Immunoblotting analysis of cyclin B1 and pY15-Cdk1 at 0 and 2 h after IBMX release in siCTL- or siMDC1-injected oocytes. D, E Levels of pY15-Cdk1 and cyclin B1 were normalized to β-actin, quantified, and expressed in arbitrary unit (a.u.). Data are expressed as the mean ± SEM from three independent experiments. *** P < 0.0001