Fig. 5.

MED12 inhibits apoptosis in glioblastoma: substrate based caspase 3/7 glo assay was performed to check for apoptosis. a, b Graph showing fold change in relative caspase 3/7 activity in cells having MED12 over-expression and knockdown in a A172 cells b T98G cells. c FACS based detection of annexin-V externalisation was checked in A172 cells post MED12 knockdown. d, e Western blotting was performed to detect cleaved PARP protein levels in A172 and T98G cells having d MED12 knockdown and e having MED12 over-expression. The actin blot was run on a different gel. The western blotting experiment was performed in duplicates. The graphical data points represent mean ± SD of at least three independent experiments (*represents p value < 0.05 and ** represents p value < 0.001). Error bars denote ± SD