Fig. 1.
Ablation of RapGEF2 protein expression in hippocampal CA1, DG and BLA. a Representative immunohistochemical images with RapGEF2 antibody (NNLE-2) for hippocampal subregions (CA1, DG and CA3) from flox and cKO mice at 5-weeks old (left panel) and 10 ~ 20 weeks old (right panel). Scale bar: 100 µm. b RapGEF2 immunoreactive (IR) signals in hippocampal CA1, DG and CA1 from cKO mice (10 ~ 20 weeks old) were quantified with NIH Image J and compared to RapGEF2 IR signals from flox mice. The result indicated a significant reduction in RapGEF2 levels in the CA1 and DG hippocampal subregions, but not in the CA3 subregion. N = 4 ~ 5 for animal number in each group. Student’s t-test for each region, **p < 0.001. c Western blots using protein lysates from hippocampal subregions of flox and cKO mice showed similar results that RapGEF2 was downregulated in CA1 and DG. N = 3 ~ 7 for animal number in each group, Student’s t-test for each region, **p < 0.001. d RapGEF2 immunoreactive (IR) signals in Amygdala from flox and cKO mice (10 ~ 20 weeks old) showed that RapGEF2 expression in BLA was significantly reduced in BLA, but not in CeA. Quantative assessment of RapGEF2 levels in BLA of wild-type and Camk2α-cre+/-::RapGEF2fl/fl mice has been previously reported (see Fig. 4B in [32])