Fig. 4.

CENPA inhibited PLA2R1 expression through facilitating PLA2R1 promoter methylation. A, B The potential interaction between CENPA and DNMT1 was validated by Co-IP assay. C, D PLA2R1 protein level was determined with Western blot assay. E MSP assay was employed to examine PLA2R1 methylation in MCF-7 cells. F ChIP assay was used to measure DNMT1 enrichment on PLA2R1 promoter. G–I PLA2R1 mRNA and protein levels were examined by RT-qPCR and Western blot analysis. N = 6. The data have been presented as mean ± SEM. **P < 0.01