Figure 3.

Artery-derived HSCs are functional in vivo upon transplantation

(A and B) Arteries were lineage-traced by administering 4OHT to E8.5 Cx40-CreERT2; Ai6 (ZsGreen reporter) embryos. B6, C57BL/6 mouse.

(C and D) ZsGreen+ E16.5 fetal liver HSCs were (B) analyzed by flow cytometry and (C and D) transplanted into lethally irradiated primary recipient mice. 1–4 months post transplantation, flow cytometry was performed to quantify ZsGreen+ (C) peripheral blood cells and (D) bone marrow HSCs in primary recipients.

(E and F) Bone marrow from primary recipient mice was transplanted into lethally irradiated secondary recipient mice. 1–4 months post transplantation, flow cytometry was performed to quantify ZsGreen+ (E) peripheral blood and (F) bone marrow HSCs in secondary recipients.

Data depict the mean ± SEM. Each dot represents a single mouse.

Related to Figure S4.