Figure 5.

Differentiation of hPSCs into hemogenic ECs and subsequently HOXA+ HLF+ hematopoietic progenitors

(A) Day-7 hemogenic ECs generated from (Ai) RUNX1-mOrange¹²⁶ or (Aii) wild-type hPSCs, profiled by flow cytometry or immunostaining, respectively. Scale bars, 50 μm.

(B) scRNA-seq of hPSC-derived day-7 hemogenic ECs. Entire population is shown, without preselecting cells.

(C) Individual differentiation factors were withheld during differentiation of day-4 hPSC-derived artery ECs into hemogenic ECs over 3 days (Ci), followed by qPCR of day-7 hemogenic ECs (Cii) and day-10 hematopoietic progenitors derived from these hemogenic ECs (Ciii and Civ).

(D) Summary (this study).

(E) Flow cytometry (Ei and Eii) and absolute number/cell yield (Eiii) of day-10 hPSC-derived hematopoietic progenitors. Each dot: independent experiment.

(F) Individual differentiation factors were withheld during differentiation of day-7 hPSC-derived hemogenic ECs into hematopoietic progenitors over 3 days, followed by qPCR of day-10 hematopoietic progenitors.

(G) scRNA-seq of differentiated hPSCs. Colors in the left-most panel indicate differentiation day.

Histograms depict the mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01; n.s., not significant.

Related to Figure S6.