Figure 7.
hPSC-derived HLF+ HOXA+ hematopoietic progenitors generate lymphoid, myeloid, and erythroid cells in vitro
(A) Summary (this study).
(B) Day-10 hPSC-derived hematopoietic progenitors were differentiated into myeloerythroid cells (Bi), erythroid cells (Bii), or macrophages (Biii). Macrophage number/yield per input progenitor is also shown.
(C–E) NK cells differentiated from day-10 hPSC-derived hematopoietic progenitors or cord blood CD34+ HSPCs. (C and D) Flow cytometry analysis, with subgating on indicated populations. (Ei) NK cell number/yield generated per input progenitor. (Eii) Live imaging of NK cells killing fluorescent OP9-DLL4-IRES-GFP cells.
(F and G) T cells differentiated from wild-type H7 hESCs, iLC13-F1 T-hiPSCs, or cord blood CD34+ HSPCs. Flow cytometry analysis with pregating on indicated populations (e.g., CD45+), and T cell number/yield generated per input progenitor.
(H) B cells differentiated from day 10 hPSC-derived hematopoietic progenitors or cord blood CD34+ HSPCs. (Hi–Hiii) Flow cytometry analysis and (Hiv) B cell number/yield generated per input progenitor.
(I) H7 AkaLuciferase-expressing hPSC-derived day-10 hematopoietic progenitors were intrafemorally transplanted into NSG mice, followed by (Ii) bioluminescent imaging and (Iii) flow cytometry.
(J) Developmental model (this study).
Histograms depict the mean ± SEM.