Fig. 6.

Ferroptotic damage mediates sepsis-induced ALI in vivo. Sham- or CLP-operated mice were pre-treated with or without ferrostatin-1 (1 mg/kg body weight, intraperitoneal injection for two consecutive days; n = 20/group). A Percent survival of mice in each group was determined using Kaplan–Meier method. At 24 h after the CLP surgery, B representative HE images of lung tissues from each group, scale bar: 100 μm; C lung injury was scored and compared between the groups; D–E cell death in lung tissues examined by TUNEL staining; F–G the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 were measured by ELISA from serum F and BALF G of indicated groups of mice; H–M lung tissues from indicated groups were measured for lung W/D weight ratio (H), MPO activity (I), total cells in BALF (J), iron (K), total glutathione (L), and MDA (M) levels. *P < 0.05, **P < 0.01 and ***P < 0.001