Fig. 8.

AUF1 depletion exacerbates CLP-induced ferroptosis in lung tissues. Sham or CLP operation was performed in AUF1 wildtype (WT) or knockout (KO) mice (n = 20/group). At 24 h after the surgery, A–C lung tissues from indicated groups were measured for iron (A), total glutathione (B), and MDA (C) levels; D–E the expression levels of GPX4, ACSL4, SLC7A11 and SLC11A2 in lung tissues by RT-PCR D and western blot (E); F–G the expression levels of NRF2 and ATF3 in lung tissues were tested by western blot F and RT-PCR (G). *P < 0.05, **P < 0.01 and ***P < 0.001