Fig. 4.

DHX9 increases the capability of migration and invasion in colorectal cancer cells. A-B Representative photographs of the wound healing scratch assay from stable DHX9-overexpression or -silenced HCT116 (A) and HCT8 (B) cells (The culture properties of COLO205 cells are mixed, adherent and suspension, which were difficult for wound healing scratch assay). Scale bar, 100 μm. C-E CRC cells with untreated, vector-transfected, stably expressing DHX9-encoding constructs or shRNAs against DHX9 were subjected to transwell chamber assays. Representative images for HCT116, HCT8, and COLO205 cells and quantitative analysis from three random microscopic fields were shown in C, D, E, respectively. Scale bar, 100 μm. ns, not significant; ***P < 0.001. F–H Control, vector-transfected, stably expressing DHX9-encoding constructs or shRNAs against DHX9 CRC cells were underwent matrigel invasion chamber assays. Representative images for HCT116, HCT8, and COLO205 cells and quantitative analysis from three random microscopic fields were shown in F, G, H, respectively. Scale bar, 100 μm. ns, not significant; ***P < 0.001. Data in all bar graphs were represented as mean ± SEM and were assessed by one-way ANOVA, post hoc intergroup comparisons, Tukey’s test