Fig. 4.

Skeletal muscle unloading does not change circadian clock, but mechanosensor proteins. Relative protein expression profiles of a CLOCK, b BMAL, c ILK, d KIND2, and e TLN2 between CTR and HS mice in GAS and SOL muscles. Expression is relative to the expression of GAPDH. Statistical analysis used was two-way ANOVA with Sidak’s multiple comparison post hoc test on the following number of animals: GAS 10 CTR and 10 HS animals, SOL 10 CTR and 10 HS animals. *p value < 0.05; ***p value < 0.001; ^#p value = 0.05–0.1. f Representative western blot bands of CLOCK, BMAL1, ILK, KIND2, and TLN2