Fig. 6.

Knockdown of either Ilk1 or Fermt2 in C2C12 myotubes alters the expression of mechanosensor, molecular, and metabolism-controlling clock genes. Relative gene expression profiles of a Ilk1, b Fermt2, Clock, Bmal1, Pfkm, Hk2, Mfn2, Nrf1 and Opa1 between Ilk1 SiRNA-treated (n = 6) and control SiRNA-treated (n = 6) C2C12 myotubes. Relative gene expression profiles of c Fermt2, d Ilk1, Clock, Bmal1, Pfkm, Hk2, Mfn2, Nrf1 and Opa1 between Fermt2 siRNA-treated (n = 6) and control siRNA-treated (n = 6) C2C12 myotubes. Expression is relative to the housekeeping gene Rpl41. Statistical analysis used was Student’s t test. *p value < 0.05; **p value < 0.01; ***p value < 0.001