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. 2020 Mar 18;78(1):287–298. doi: 10.1007/s00018-020-03499-7

Fig. 2.

Fig. 2

Effect of Map3k12 (Dlk) silencing in beta-cell proliferation, basal beta-cell death, Ccnd1 and Ccnd2 mRNA levels. The expression of the Map3k12 (Dlk) was measured in dispersed islet cells from 10-day-old rats by a Western Blotting experiments in dispersed islet cells of newborn rats of 10 days old, which were transfected with control siRNA (siCtl; siRNA directed against GFP) or siRNAs against Map3k12 (Dlk) (siDlk) for 48 h. beta cell proliferation was quantified by determining the percentage of insulin + (ins+) and b Ki67+ cells (n = 4) or c BrdU+ (n = 3). d Effect of siDLK on the expression of Ccnd1 and Ccnd2 in the islet cells of 10-day-old rats. The mRNA levels were normalized to those of Tbp and were expressed as % changes. The data are presented as means ± SEM of 5 independent experiments performed in triplicate as determined by ANOVA, (*p < 0.05, ***p < 0.001). e Beta-cell death was assessed by scoring the percentage of insulin positive cells labelled with TUNEL (n = 3). f insulin secretion after 45 min exposure to 2 or 20 mM glucose (n = 4). The data are the mean ± SEM of 3 independent experiments performed in triplicate (***p < 0.001, by ANOVA)