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. 2020 Mar 18;78(1):287–298. doi: 10.1007/s00018-020-03499-7

Fig. 4.

Fig. 4

Effect of Mapk10 (Jnk3) silencing in beta-cell proliferation and Ccnd1 and Ccnd2 mRNA levels a Newborn rat islet cells were transfected with control siRNA (siCtl; siRNA directed against GFP) or siRNAs against Jnk3 for 48 h and Jnk3 mRNA levels were measured by RT-qPCR. beta cell proliferation was quantified by determining the percentage of insulin + (ins+) and b Ki67 + cells (n = 3) and c BrdU. d Effect of siJnk3 on the expression of indicated genes in the islet cells of 10-day-old rats. Data are the means ± SEM of 3 independent experiments performed in triplicate (*p < 0.05, ***p < 0.001; ANOVA)