Fig. 5.

Measurement of islets Map3k12 (Dlk), Ccnd1 and Ccnd2 expression changes in mice model of pregnancy and obesity. a The expression of Map3k12 (Dlk), Mapk10 (Jnk3), Ccnd1 and Ccnd2, was measured by RT-qPCR in the islets of female rats at indicated gestational days 10 (G10), 12 (G12), 14 (G14), and 3 days post-partum (P3). The results are expressed as % of the level in (Ctl). b Measurement of Map3k12 (Dlk) and Mapk10 (Jnk3) and c Ccnd1 and Ccnd2 in islets isolated from ob/ob mice at the indicated ages and of age-matched controls. The data represent the mean ± SEM of 4 animals per group. (Significantly different non-pregnant rats ***p < 0.001; **p < 0.01; *p < 0.05 by Dunnett’s post-hoc test). NS Not significant