Fig. 2.

Extended activity screening of AA5_2 enzymes. Measurements were performed in triplicate at room temperature in 100 mM buffer, using the HRP/ABTS assay. Activities were monitored using 300 mM for carbohydrates, polyols, diols, and primary alcohols, 30 mM for benzyl alcohol and galactitol, 2.5 mM for aldehydes, aryl alcohols, and furans. Reactions were started with the addition of 30 pmol–0.26 nmol of purified enzyme