Fig. 1.

Isolation and characterization of cochlear tissue-derived sEVs. a The workflow for isolating cochlear tissue-derived sEVs by ultracentrifugation. b TEM of cochlear sEVs. Scale bar = 100 nm. c Western blotting of cochlear tissue lysate (TL) and sEV samples. CD63, CD9, and Tsg101 were used as sEV markers, and EEA1, Rab7, GAPDH, Synapsin-1, and VGLUT3 from other organelles were used as negative markers. d NTA of cochlear sEVs from P3, P7, P14, and P21 mice. e Immunofluorescent staining of CD63 and CD9 (red) in the P3 cochlea. Myo7a (green) and Sox2 (blue) were used as HC and SC markers, respectively. OHC, outer hair cell. IHC, inner hair cell. DC, Deiters’ cell. IPC, inner pillar cell. OPC, outer pillar cell. IPhC, inner phalangeal cell. Scale bar = 20 μm